Iba1 antibody (AA 1-146)

Catalog No. ABIN7477371

This Mouse Monoclonal antibody specifically detects Iba1 in IHC. It exhibits reactivity toward Human.

Quick Overview for Iba1 antibody (AA 1-146) (ABIN7477371)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Iba1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-955M

Product Details anti-Iba1 Antibody

Binding Specificity: AA 1-146

Immunogen: Purified recombinant human AIF1 protein (around aa 1-146) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: An at least moderate to strong cytoplasmic AIF1 immunostaining should be seen in mucosal macrophages.

Negative Control: Colon: AIF1 immunostaining should be absent in all non-inflammatory cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: AIF1

Background: Allograft inflammatory factor 1 (AIF1), balloon angioplasty responsive transcription (BART1), IBA1, Interferon responsive transcript 1, Ionized calcium-binding adapter molecule 1, IRT1, Microglia response factor (MRF1),AIF1 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P55008

Pathways: Smooth Muscle Cell Migration